intengo ephansi yefektri i-Alkaline ProteinaseK CAS 39450-01-6
Sizibophezele ekunikezeni izinga lokuncintisana, imikhiqizo nezixazululo ezisezingeni eliphezulu, kanye nokulethwa okusheshayo kwe-Alkaline ephansi efektriI-ProteinaseK CAS 39450-01-6Besilokhu sifuna ngobuqotho ukubambisana nabathengi emhlabeni wonke. Sikholelwa ukuthi sizokwanelisa. Siphinde samukele ngemfudumalo abathengi ukuthi baye enhlanganweni yethu bathenge imikhiqizo yethu.
Sizibophezele ekunikezeni izinga lokuncintisana, imikhiqizo nezixazululo ezisezingeni eliphezulu, kanye nokulethwa okusheshayo kwe-I-China Alkaline ProteinaseK kanye ne-39450-01-6Ngenxa yekhwalithi enhle namanani afanelekile, izimpahla zethu zithunyelwe emazweni nasezifundeni ezingaphezu kwe-10. Silangazelela ukubambisana nawo wonke amakhasimende avela ekhaya nakwamanye amazwe. Ngaphezu kwalokho, ukwaneliseka kwamakhasimende kuyinto esiyiphishekelayo phakade.
| Uketshezi lweNombolo 1 | |
| Umsebenzi othize weprotheni>800U/mL, ukuhlushwa kungu-20mg/mL, akukho nsalela ye-Nickase | |
| Impuphu yenombolo 2 | |
| Ukubukeka | Impuphu emhlophe e-lyophilized |
| Ubumsulwa be-electrophoretic | ≥95% |
| Umsebenzi we-enzyme | ≥30U/mg |
| Izinsalela ze-Nucleic acid | Okungabonakali |
| I-Deoxyribonuclease residue | Okungabonakali |
| Izinsalela ze-Ribonuclease | Okungabonakali |
| Inombolo Yenqola | G1205-10ML |
| Isitoreji | Uma igcinwe ku--20 °C, umkhiqizo ugcina umsebenzi okungenani unyaka owodwa, ukuthuthwa kwesikhwama seqhwa. |
I-Proteinase Kiyi-serine protease engowomndeni we-subtilisin enomsebenzi ophumelelayo we-enzymatic kanye nokucaciswa kwe-substrate ebanzi, ehlukanisa ngokukhethekile izibopho ze-ester nama-peptide eduze kwe-C-terminus yama-amino acids e-hydrophobic, ama-amino acids aqukethe i-sulfur, kanye nezibopho ze-aromatic amino acid, ezivame ukusetshenziselwa ukonakalisa amaprotheni ukukhiqiza ama-peptide amafushane. I-Proteinase K inesici se-catalytic triad ejwayelekile i-Asp39-His69-Ser224 ehlukile kuma-serine proteases futhi inezindawo ezimbili zokubopha ze-Ca2+ ezizungeze isikhungo esisebenzayo ukwandisa ukuzinza kwayo nokugcina umsebenzi we-enzymatic ophezulu ngaphansi kwezimo eziningi.
1. Ikhithi yokuxilonga izakhi zofuzo.
2. Amasethi okukhipha i-RNA kanye ne-DNA.
3. Khipha izingxenye ezingezona amaprotheni ezicutshini bese wonakalisa ukungcola kwamaprotheni (njengokulungiselela imijovo ye-DNA kanye ne-heparin).
4. Ukulungiswa kwe-Chromosomal DNA ye-Pulse Electrophoresis.
5. Ukuqothula kweNtshonalanga.
6. Ukuthuthukiswa nokukhiqizwa kwama-reagent e-albhamuin e-glycated enzyme emkhakheni wokuxilongwa kwe-in vitro.
1. 30mg/ibhodlela 1g/ibhodlela noma 100g/ibhodlela
2. 1kgs/isikhwama sefoyili
I-Proteinase K iyi-protease ye-serine eqinile enezici ezibanzi ze-substrate. Yonakalisa amaprotheni amaningi esimweni sayo sendawo ngisho nalapho kukhona izihlanzi. Ayisebenzi ngama-ion ensimbi, ama-chelating agents (isibonelo, i-EDTA), ama-reagents e-sulfhydryl, noma ngama-tripsin noma ama-chymotrypsin inhibitors. Izinzile ebangeni elibanzi le-pH (4–12.5), ngomsebenzi omuhle kakhulu ku-pH 6.5–9.5. Umsebenzi ungavuselelwa ngokwengeza ama-denaturing agents (SDS kanye ne-Urea). Ukuncipha okusheshayo kwe-enzyme kwenzeka ekushiseni okungaphezu kuka-70°C. Ukuguqulwa kwe-enzyme kwenzeka ngokwandayo ku-alkaline pH. Kodwa-ke, i-Proteinase K ayisebenzi ngokuphelele ngokuzihlukanisa. Ezinye izingcezu ze-enzyme ziyaqhubeka nokugcina umsebenzi wazo ophelele we-proteolytic, ngisho nangemva kokuzihlukanisa okukhulu. I-Proteinase K ivame ukusetshenziswa ezinhlelweni ze-molecular biology ukugaya amaprotheni angafuneki, njenge-nucleases kumalungiselelo e-DNA noma e-RNA avela kuma-microorganisms, amaseli akhuliswe, kanye nezitshalo. I-enzyme ivame ukusetshenziswa ku-50–200 ug/ml kumalungiselelo e-nucleic acid ku-pH 7.5–8.0 kanye no-37~55oC. Izikhathi zokufukamela ziyahlukahluka kusukela kumaminithi angu-30 kuya emahoreni angu-18.
I-serine protease eqinile futhi esabela kakhulu; esetshenziselwa ukuhlukaniswa kwamaprotheni kanye ne-nucleic acid. I-Proteinase K isetshenziswa ekuhlanzweni kwe-RNA kanye ne-DNA ezicutshini noma emigqeni yamaseli.
Sizibophezele ekunikezeni izinga lokuncintisana, imikhiqizo nezixazululo ezisezingeni eliphezulu, kanye nokulethwa okusheshayo kwe-Alkaline ProteinaseK eshibhile efektri.I-CAS39450-01-6Besilokhu sifuna ngobuqotho ukubambisana nabathengi emhlabeni wonke. Sikholelwa ukuthi sizokwanelisa. Siphinde samukele ngemfudumalo abathengi ukuthi baye enhlanganweni yethu bathenge imikhiqizo yethu.
intengo ephansi yefektriI-China Alkaline ProteinaseK kanye ne-39450-01-6Ngenxa yekhwalithi enhle namanani afanelekile, izimpahla zethu zithunyelwe emazweni nasezifundeni ezingaphezu kwe-10. Silangazelela ukubambisana nawo wonke amakhasimende avela ekhaya nakwamanye amazwe. Ngaphezu kwalokho, ukwaneliseka kwamakhasimende kuyinto esiyiphishekelayo phakade.
