intengo ephansi yefekthri I-Alkaline ProteinaseK CAS 39450-01-6

Sizibophezele ekunikezeni izinga lokuncintisana, imikhiqizo eyingqayizivele nezisombululo zekhwalithi ephezulu, futhi njengokulethwa okusheshayo ngentengo ephansi yefekthri I-AlkalineI-ProteinaseK CAS 39450-01-6, Besilokhu sifuna phambili ngobuqotho ukubambisana nabathengi yonke indawo emhlabeni wonke. Sikholwa ukuthi sizokwanelisa. Siphinde futhi sibamukele ngemfudumalo abathengi ukuthi baye enhlanganweni yethu futhi bathenge imikhiqizo yethu.
Sizibophezele ekunikezeni izinga lokuncintisana, imikhiqizo eyingqayizivele nezisombululo zekhwalithi ephezulu, futhi njengokulethwa okusheshayoI-China Alkaline ProteinaseK kanye ne-39450-01-6, Ngenxa yekhwalithi enhle nezintengo ezinengqondo, okuthengiswayo kwethu kuthunyelwe emazweni angaphezu kuka-10 nasezifundeni. Sibheke ngabomvu ukubambisana nawo wonke amakhasimende asekhaya nawaphesheya. Ngaphezu kwalokho, ukwaneliseka kwamakhasimende kuwumsebenzi wethu waphakade.

Amaprotheni Ki-serine protease eyingxenye yomndeni we-subtilisin esebenza ngempumelelo ye-enzymatic kanye nokucaciswa kwe-substrate ebanzi, ekhetha ngokukhethekileyo ukubola amabhondi e-ester nama-peptide eduze kwe-C-terminus yama-amino acid e-hydrophobic, ama-amino acid aqukethe isulfure, namabhondi ama-amino acid anephunga elimnandi. ngokuvamile asetshenziselwa ukwehlisa amaprotheni ukukhiqiza ama-peptide amafushane. I-Proteinase K inesici se-catalytic triad evamile i-Asp39-His69-Ser224 ehlukile kuma-serine protease futhi inezindawo ezimbili ezibophayo ze-Ca2+ esikhungweni esisebenzayo ukuze kukhuliswe ukuzinza kwayo nokugcina umsebenzi ophezulu we-enzymatic ngaphansi kobubanzi obubanzi bezimo.

1. Ikhithi yokuxilongwa kwezakhi zofuzo.
2. I-RNA kanye ne-DNA extraction kits.
3. Khipha izingxenye ezingezona amaprotheni ezicutshini futhi wehlise ukungcola kwamaprotheni (njengokulungiswa kwemigomo ye-DNA ne-heparin).
4. Ukulungiswa kwe-Chromosomal DNA ye-Pulse Electrophoresis.
5. Ukubhula kwaseNtshonalanga.
6. Ukuthuthukiswa nokukhiqizwa kwama-enzymatic glycated albumin reagents emkhakheni we-in vitro diagnostics.

1. 30mg/ibhodlela 1g/ibhodlela noma 100g/ibhodlela
2. 1kgs/isikhwama se-foil

I-Proteinase K iyi-serine protease ezinzile enokucaciswa okubanzi kwe-substrate. Yehlisa isithunzi samaprotheni amaningi endaweni yendabuko ngisho nalapho kukhona izinto zokuhlanza. Ayenziwe isebenze ngama-ion ensimbi, ama-chelating agents (isibonelo, i-EDTA), ama-reagents e-sulfhydryl, noma ama-tripsin noma ama-chymotrypsin inhibitors. Izinzile phezu kwebanga elibanzi le-pH (4–12.5), inomsebenzi ofanele ku-pH 6.5–9.5. Umsebenzi ungagqugquzelwa ngokwengezwa kwama-ejenti e-denaturing (i-SDS ne-Urea). Ukushintsha ngokushesha kwe-enzyme kwenzeka ekushiseni okungaphezu kuka-70°C. I-autolysis ye-enzyme kwenzeka ngokuya nge-pH ye-alkaline. Kodwa-ke, iProteinase K ayenziwanga isebenze ngokuphelele ngokuzihlaziya. Ezinye izingcezu ze-enzyme ziyaqhubeka nokugcina umsebenzi wazo ophelele we-proteolytic, ngisho nangemva kokuhlaziywa okuzenzakalelayo okubanzi. I-proteinase K ivamise ukusetshenziswa ezinhlelweni zebhayoloji yamangqamuzana ukuze kugaywe amaprotheni angafuneki, njengama-nuclease ku-DNA noma amalungiselelo e-RNA avela kumagciwane, amaseli akhulisiwe, nezitshalo. I-enzyme ivame ukusetshenziswa ku-50-200 ug / ml kumalungiselelo e-nucleic acid ku-pH 7.5-8.0 no-37 ~ 55oC. Izikhathi zokufukamela ziyahlukahluka kusuka emaminithini angama-30 kuye kwamahora ayi-18.

I-serine protease ezinzile futhi esebenza kakhulu; esetshenziselwa amaprotheni kanye ne-nucleic acid isolation.I-Proteinase K isetshenziselwa ukuhlanzwa kwe-RNA ne-DNA ezicutshini noma emigqeni yamaseli.

Sizibophezele ekunikezeni izinga lokuncintisana, imikhiqizo eyingqayizivele nezisombululo zekhwalithi ephezulu, futhi njengokulethwa okusheshayo ngentengo ephansi yefekthri I-Alkaline ProteinaseKI-CAS39450-01-6, Besilokhu sifuna phambili ngobuqotho ukubambisana nabathengi yonke indawo emhlabeni wonke. Sikholwa ukuthi sizokwanelisa. Siphinde futhi sibamukele ngemfudumalo abathengi ukuthi baye enhlanganweni yethu futhi bathenge imikhiqizo yethu.
intengo ephansi yefekthriI-China Alkaline ProteinaseK kanye ne-39450-01-6, Ngenxa yekhwalithi enhle nezintengo ezinengqondo, okuthengiswayo kwethu kuthunyelwe emazweni angaphezu kuka-10 nasezifundeni. Sibheke ngabomvu ukubambisana nawo wonke amakhasimende asekhaya nawaphesheya. Ngaphezu kwalokho, ukwaneliseka kwamakhasimende kuwumsebenzi wethu waphakade.