Umklamo Wobungcweti Wekhwalithi Ephakeme Yeprotheyini K Inombolo ye-CAS 39450-01-6 ngentengo engcono kakhulu
Sivame ukusebenza njengeqembu elibonakalayo ukuqinisekisa ukuthi sikunikeza okuhle kakhulu kanye nentengo yokuthengisa engcono kakhulu yekhwalithi ephezulu yokuklama kochwephesheI-Proteinase K Inombolo ye-CAS 39450-01-6ngentengo engcono kakhulu, Ukuze uthole ulwazi olwengeziwe kanye namaqiniso, sicela ukhulume nathi ngokushesha okukhulu!
Sivame ukusebenza njengeqembu elibonakalayo ukuqinisekisa ukuthi sikunikeza okuhle kakhulu kanye nentengo yokuthengisa engcono kakhuluI-albhamu ye-China Tritirachium Limber kanye ne-Molecular Biology, Ukulawulwa kwekhwalithi okuqinile kwenziwa kuyo yonke isixhumanisi senqubo yonke yokukhiqiza. Sithemba ngobuqotho ukusungula ukubambisana okunobungane nokuzuzisayo nawe. Ngokusekelwe ezixazululweni zekhwalithi ephezulu kanye nensizakalo ephelele yangaphambi kokuthengisa/ngemuva kokuthengisa, umbono wethu, amanye amaklayenti abesebenzisana nathi iminyaka engaphezu kwemi-5.
| Uketshezi lweNombolo 1 | |
| Umsebenzi othize weprotheni>800U/mL, ukuhlushwa kungu-20mg/mL, akukho nsalela ye-Nickase | |
| Impuphu yenombolo 2 | |
| Ukubukeka | Impuphu emhlophe e-lyophilized |
| Ubumsulwa be-electrophoretic | ≥95% |
| Umsebenzi we-enzyme | ≥30U/mg |
| Izinsalela ze-Nucleic acid | Okungabonakali |
| I-Deoxyribonuclease residue | Okungabonakali |
| Izinsalela ze-Ribonuclease | Okungabonakali |
| Inombolo Yenqola | G1205-10ML |
| Isitoreji | Uma igcinwe ku--20 °C, umkhiqizo ugcina umsebenzi okungenani unyaka owodwa, ukuthuthwa kwesikhwama seqhwa. |
I-Proteinase Kiyi-serine protease engowomndeni we-subtilisin enomsebenzi we-enzyme ophumelelayo kanye nokucaciswa kwe-substrate ebanzi, ehlukanisa ngokukhethekile izibopho ze-ester nama-peptide eduze kwe-C-terminus yama-amino acids e-hydrophobic, ama-amino acids aqukethe i-sulfur, kanye nezibopho zama-amino acids anuka kamnandi, ezivame ukusetshenziselwa ukonakalisa amaprotheni ukukhiqiza ama-peptide amafushane.I-Proteinase Kinesici se-catalytic triad ejwayelekile i-Asp39-His69-Ser224 eyingqayizivele kuma-serine proteases futhi inezindawo ezimbili zokubopha ze-Ca2+ ezizungeze isikhungo esisebenzayo ukwandisa ukuzinza kwayo nokugcina umsebenzi ophezulu we-enzyme ngaphansi kwezimo ezahlukahlukene.
1. Ikhithi yokuxilonga izakhi zofuzo.
2. Amasethi okukhipha i-RNA kanye ne-DNA.
3. Khipha izingxenye ezingezona amaprotheni ezicutshini bese wonakalisa ukungcola kwamaprotheni (njengokulungiselela imijovo ye-DNA kanye ne-heparin).
4. Ukulungiswa kwe-Chromosomal DNA ye-Pulse Electrophoresis.
5. Ukuqothula kweNtshonalanga.
6. Ukuthuthukiswa nokukhiqizwa kwama-reagent e-albhamuin e-glycated enzyme emkhakheni wokuxilongwa kwe-in vitro.
1. 30mg/ibhodlela 1g/ibhodlela noma 100g/ibhodlela
2. 1kgs/isikhwama sefoyili
I-Proteinase K iyi-protease ye-serine eqinile enezici ezibanzi ze-substrate. Yonakalisa amaprotheni amaningi esimweni sayo sendawo ngisho nalapho kukhona izihlanzi. Ayisebenzi ngama-ion ensimbi, ama-chelating agents (isibonelo, i-EDTA), ama-reagents e-sulfhydryl, noma ngama-tripsin noma ama-chymotrypsin inhibitors. Izinzile ebangeni elibanzi le-pH (4–12.5), ngomsebenzi omuhle kakhulu ku-pH 6.5–9.5. Umsebenzi ungavuselelwa ngokwengeza ama-denaturing agents (SDS kanye ne-Urea). Ukuncipha okusheshayo kwe-enzyme kwenzeka ekushiseni okungaphezu kuka-70°C. Ukuguqulwa kwe-enzyme kwenzeka ngokwandayo ku-alkaline pH. Kodwa-ke, i-Proteinase K ayisebenzi ngokuphelele ngokuzihlukanisa. Ezinye izingcezu ze-enzyme ziyaqhubeka nokugcina umsebenzi wazo ophelele we-proteolytic, ngisho nangemva kokuzihlukanisa okukhulu. I-Proteinase K ivame ukusetshenziswa ezinhlelweni ze-molecular biology ukugaya amaprotheni angafuneki, njenge-nucleases kumalungiselelo e-DNA noma e-RNA avela kuma-microorganisms, amaseli akhuliswe, kanye nezitshalo. I-enzyme ivame ukusetshenziswa ku-50–200 ug/ml kumalungiselelo e-nucleic acid ku-pH 7.5–8.0 kanye no-37~55oC. Izikhathi zokufukamela ziyahlukahluka kusukela kumaminithi angu-30 kuya emahoreni angu-18.
I-serine protease eqinile futhi esabela kakhulu; esetshenziselwa ukuhlukaniswa kwamaprotheni kanye ne-nucleic acid. I-Proteinase K isetshenziswa ekuhlanzweni kwe-RNA kanye ne-DNA ezicutshini noma emigqeni yamaseli.
Ngokuvamile sisebenza njengeqembu elibonakalayo ukuqinisekisa ukuthi sizokunikeza okuhle kakhulu kanye nentengo yokuthengisa engcono kakhulu yeProtheinase K CAS No. 39450-01-6 enentengo engcono kakhulu, Ukuze uthole ulwazi olwengeziwe namaqiniso, sicela ukhulume nathi ngokushesha okukhulu!
Umklamo WobungcwetiI-albhamu ye-China Tritirachium Limber kanye ne-Molecular Biology, Ukulawulwa kwekhwalithi okuqinile kwenziwa kuyo yonke isixhumanisi senqubo yonke yokukhiqiza. Sithemba ngobuqotho ukusungula ukubambisana okunobungane nokuzuzisayo nawe. Ngokusekelwe ezixazululweni zekhwalithi ephezulu kanye nensizakalo ephelele yangaphambi kokuthengisa/ngemuva kokuthengisa, umbono wethu, amanye amaklayenti abesebenzisana nathi iminyaka engaphezu kwemi-5.
